First order derivative spectroscopy method involves the measurement of absorbance of one drug at zero crossing point of the other drug. A new, rapid, precise, accurate and sensitive first derivative spectrophotometric method is proposed for the simultaneous estimation of pantoprazole Sodium and Ondansetron Hydrochloride in combined dosage form. Absorbance of Pantoprazole Sodium was measured at 310 nm (Zero Crossing Point of Ondansetron Hydrochloride) and Absorbance of Ondansetron Hydrochloride was measured at 331 nm (Zero Crossing Point of Pantoprazole Sodium). The drugs obeyed the Beer’s law in the concentration range of 10-50 ug/ml (r2 = 0.9997) for Pantoprazole Sodium and1-5 ug/ml ( r2 = 0.9991) Ondansetron Hydrochloride. In the proposed method,. Accuracy of the method was determined by recovery studies and the average percentage recovery was found to be 99.24 % and 99.37 % for Pantoprazole Sodium and Ondansetron Hydrochloride respectively. The LODs for Pantoprazole Sodium and Ondansetron Hydrochloride were found to be 2.69 μg/ml and 0.29 μg/ml & LOQs were found to be 8.17 μg/ml and 0.89 μg/ml respectively. Statistical analysis proves that the method is selective and sensitive for estimation of Pantoprazole Sodium and Ondansetron Hydrochloride in combined dosage form. The results were found to be within acceptance criteria according to ICH Q2 R1 guideline.
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